盐酸哌唑嗪对脱交感家兔眼葡萄膜巩膜途径的形态学研究

Morphological study of uveoscleral pathway following topical Prazosin in free-sympathetic rabbit eyes

目的从形态学探讨盐酸哌唑嗪（ＰＺ）对脱交感家兔眼（ＦＳＲＥ）葡萄膜巩膜途径（ＦＵ）的作用。方法家兔作单侧颈交感神经节切除术后第１４日在手术侧点ＰＺ滴眼液，通过前房内注入异硫氰酸荧光素牛血清白蛋白，ＰＺ点眼后每２ ｈ各处死家兔 ２只，于荧光显微镜下观察 ＦＵ各部位的荧光强度等级。结果 ＰＺ点眼后 ＦＳＲＥ眼压下降，但降压幅度低于正常家兔眼；点眼后ＦＵ各部位的荧光强度均稍强于对侧眼的相应部位，但明显弱于ＰＺ在正常家兔眼的相应部位。结论ＰＺ增加ＦＵ排出的作用在ＦＳＲＥ明显减弱，其作用部分依赖于交感神经活性，尚有未通过交感活性的其它机制。

Objective To observe the morphology of the uveoscleral pathway following topical Prazosin(PZ) in rabbit freesympathetic eyes. Methods The one lateral superior cervical ganglionectomy(SCG) were preformed on each rabbit. PZ drops were installed only on the SCG eyes on 14 days Postoperationly. Microamounts of fluorescein isothiocyanate-bovine serum albumin (FITC-BSA ) was infused into anterior chamber of rabbit eyes as the teacer,two rabbits in each group were killed at 2,4,6,8, 10, and 12 h after PZ instillation. Degress of fluorescent intensity of ciliary body, suprachoroidal space, anterior and posterior sclera, and choroid were observed under fluorescence microscopy. Results The IOP of the SCG eyes was lower after installation of PZ. But the reduced extent is below that of normal rabbit eyes; After installation of PZ,the nuorescent intensity of the ciliary body, suprachoroidal space,anterior and posterior sclera,and choroid was less stronger than the control eyes,but fader than at the same area of the normal eyes obviously. Conclusion The outflow effects of PZ drops on increasing uveoscleral pathway were obviously decreased in the SCG eyes. It depends on the activity of sympathetic nerve system partially. Besides,there are other mechanisms.