阿苯达唑免疫脂质体的制备

Preparation of albendazole immunoliposome

目的 :制备阿苯达唑免疫脂质体 ,提高阿苯达唑治疗包虫病的靶向特异性。方法 :采用注入 - p H梯度法制备了脂质体 ;接着进一步采用戊二醛法将单克隆抗体与脂质体偶联制备免疫脂质体。 结果 :脂质体的包封率为( 6 4.0 2± 3.10 ) % ,粒径 2 0～ 80 nm;用 EL ISA法检测了免疫脂质体抗体效价 ,其活性仍保留近 80 %。结论

Objective: To prepare albendazole immunoliposomes for improving targeting specificity against echinococcosis granulosus. Method: Albendazole liposomes were prepared by infusion in conjunction with pH gradient method, then coupled to monoclonal antibody by glutaraldhyde method. Results: Liposomal encapsulation efficiency was 64.02±3.10% and the average particle size was 20～80nm;enzyme linked immunosorbent assay indicated that the immunoconjugate retained up to 80% of the original antibody activity. Conclusion: Albendazole immunoliposomes may become a specific targeting drug against echinococcosis granulosus.