不同类型损伤对脊髓背角神经元的影响

Morphological Changes of Neurons in the Dorsal Horn Resulted from Different Types of Spinal Cord Injury

为了解不同类型损伤对脊髓背角神经元的影响 ,采用本室建立的SD成年大鼠脊髓挤压伤、半横断伤和全横断伤模型 ,分别于术后第 2 4h ,7d ,2 1d处死动物并设正常对照组 .取L3 节段脊髓制作 2 0 μm厚连续冰冻切片 .间隔取片行苏木精染色 .光镜下观察损伤位点尾侧端背角的形态改变 ,计数背角神经元数 .结果 :组间比较 ,挤压伤者较正常者神经元明显萎缩 ,胞体周围有空隙 ,神经元数无明显减少 (P >0 0 5 ) ,半横断伤和全横断伤者均出现大量空泡 ,未见丰满而轮廓清楚的神经元 ,残存的少数神经元明显萎缩 ,术后神经元数明显减少且以全横断伤者为甚 (P <0 0 1) .组内比较 ,3种损伤术后各时相神经元数的变化均无显著性差异 (P >0 0 5 ) .结果表明 :不同类型损伤对背角神经元存活的影响不同 ,全横断伤者最重 ,半横断伤者次之 ,挤压伤者相对较轻 .

To explore the characteristic features of morphological changes of neurons in the spinal dorsal horn result from different types of spinal cord injury, the adult Srague-Daweley fat models of crushed, hemi-sected and transected spinal cord injury established in our laboratory were used, and the intact spinal cords were as control. These rats were sacrificed after 24 hours, 7 days and 2l days of operation, and the L3, segments were removed out and sectioned continuously into sections of 20 μm in thickness. The sections were stained by hematoxylin and observed under . microscope. In addition, neurons in the dorsal horn were counted. Results: In the crushed spinal horns, bodies of neurons were atrophy, but neuron counting did not decrease markedly; in the hemi-sected and transected dorsal horn, a batch of empty cavities were presented, and neuron counting decreased greatly. The results indicated that different injuries of spinal cord resulted in different damage to neurons in the dorsal horn, i. e. the crushed one, the bad, the hemi-sected one, the worse; and the transected one, the worst.