人工合成高密度脂蛋白样颗粒测定血清胆固醇酯转运蛋白活性

Preparation of artificial HDL particle in vitro for determination of cholesterly ester transfer protein activity in serum.

目的建立血清胆固醇酯转运蛋白活性测定方法。方法用１４Ｃ标记的胆固醇酯合成高密度脂蛋白（ＨＤＬ）样颗粒作为胆固醇酯的供体，以低密度脂蛋白为受体测定血清中胆固醇酯转运蛋白（ＣＥＴＰ）活性，并对４０例健康人、４５例冠心病（ＣＨＤ）、２６例脑卒中患者血清ＣＥＴＰ活性进行分析。结果 该方法线性范围为０～３０％；高（２６％）、低（６．８％）活性批内变异系数（ＣＶ）分别为６．０％、７．５％，批间ＣＶ分别为１１．５％和１２．３％；４５例ＣＨＤ患者血清ＣＥＴＰ活性（ｘ±ｓ）为１７．６％±５．４％，２６例脑卒中患者为１５．２％±３．８％，均明显高于健康对照（１２．７％±３．９％）（Ｐ＜０．０１）。结论该法简便快速、精密度较高，为临床及科研工作中测定ＣＥＴＰ活性提供了一种有效的方法。

Objective To establish a method for determining of cholesterly ester transfer protein(CETP) activity in serum. Methods The assay was based on14C cholesterly ester-labeled artificial HDL particle as donor and purified LDL as the acceptor for the cholesterly ester.The CETP activity was analyaed in coronary heart disease (CHD), stroke patients and control subjects.Results The assay was linear from 0 to 30 %,With high and low activity, the within-assay CV was 6 .0% and 7. 5 % respectively, and the day-to-day CV 11 .5% and 12.3% .CETP activity in 45 CHD patients(17 .6% ±5 .4% ) and 26 stroke patients (15 .2% ± 3 .8% ) were significantly higher than that of the control group(10. 7% ± 3. 9% ) (P<0 .01 ) .Conclusion It is an easy, rapid and precise method. The method can be used for determining CETP activity in clinical research