Bcl-2及Bax基因蛋白在听神经瘤组织中的表达及意义

Expression of Bcl-2/Bax Oncogene Protein in Acoustic Neuroma Tissues and Its Significance

目的 :评价Bcl 2及Bax基因蛋白在听神经瘤组织中的表达及意义。方法 :取库存石蜡包埋组织标本 ,40例听神经瘤分作复发组、囊变组及对照组 ,采用SABC法免疫组化半定量检测Bcl 2及Bax蛋白在听神经瘤组织中的表达。结果 :Bcl 2阳性者 2 7例 ,多见于AntoniA区 ;Bax阳性者 2 6例 ,多见于AntoniB区。不同组间Bcl 2与Bax表达的相对构成有差别 (χ2 =6 64 ,P <0 0 5 )。结论 :Bcl 2

Aim:To evaluate the level of two kinds of oncogene proteins Bcl 2/Bax expression within paraffin embedded acousitc neuroma (AN) tissues and its significance. Methods:40 cases of selected archival AN materials were classified as recurrence (10 cases), cystic degeneration (10 cases) and common contrast (20 cases). 3 groups based on the different clinical biological behavior of ANs separately, and were detected for Bcl 2/Bax protein expression by SABC immunohistochemical technique and semi quantitative scales.Results:Of all cases, 27 were positive for Bcl 2 (67 5%), mainly within Antoni A zone and stained heavily and widespread; 26 were positive for Bax (65%), major within Antoni B zone and stained lightly and scattered. According to the relative expression level (REL) of Bcl 2/Bax, there were differences with the REL construction rate of Bcl 2 versus Bax proteins among groups (χ 2=6 64, P <0 05), that is, Bcl 2>Bax were recurrence>common contrast>cystic degeneration. Conclusion:There is an active expression of apoptosis related protein within paraffin embedded AN tissues. Singly, neither Bcl 2 nor Bax is related to clinical biological behavior of ANs. The construction rate of Bcl 2/Bax REL, however, would be related to it.