大兴安岭地区人群埃立克体感染的调查

Investigations on Human ehrlichia infectious people in Daxingan Mountains

目的 了解我国大兴安岭地区可疑人群是否存在人埃立克体感染。方法 用 16SrRNA基因特异引物进行半巢式多聚酶链反应及基因序列分析技术 ,检测大兴安岭地区的一些人血标本中查菲埃立克体 (Ehrlichiachaffeensis,EC)和人粒细胞埃立克体 (humangranulocyticehrlichia)的16SrRNA基因 ,并进行序列分析 ,将测出的序列与GenBank中注册的基因序列进行最大同源性比较。结果 从蜱咬后出现发热等病症的 35例病人和近一年内有蜱咬史的林场工人血中检测到这两种埃立克体的 16SrRNA基因片段 ;对 1份可疑埃立克体病人血标本进行了人粒细胞埃立克体 16SrRNA全基因扩增 ,测出的序列与美国 1株人粒细胞埃立克体的该基因仅差 1个碱基。

Objective In order to investigate the prevalence of Human ehrlichia infectious people in Daxingan Mountains. Methods Using PCR detection,followed by sequence analysis to detect EC and Human granulocytic ehrlichia from people in Daxingan Mountains applying the 16S rRNA gene primers of the two agents. Results Specific 16S rRNA gene sequences of EC and HGE agent were amplified from human blood specimens of tick bitted patients and woodsmen. The detected HGE agent 16S rRNA gene ( 1 433 bp) amplified from a blood specimen of a tick bitted patient differed in only one base compared with that of an American HGE agent strain. Conclusion It was considered that Human ehrlichia infectious people did exist in Daxingan Mountains.