菟丝子多糖H3的研究

STUDIES ON THE POLYSACCHARIDE H3 OF CUSCUTA CHINENSIS LAM

目的 研究从中药菟丝子 (CuscutachinensisLam .)种子中提取分离所得的酸性纯多糖H3的结构特征。方法 利用化学方法 (糖组分分析、甲基化分析、糖醛酸还原和部分酸水解等 )和光谱分析方法 (1HNMR谱、13CNMR谱、IR谱等 )对其结构进行了研究。结果 H3的分子量大于 1 0× 10 6,糖组分分析显示其是由阿拉伯糖、鼠李糖、半乳糖和半乳糖醛酸组成的 ,甲基化分析 ,部分酸水解和NMR光谱分析进一步揭示H3分子中各糖残基的连接方式及次序。结论 H3为一多分枝的结构复杂的杂多糖 ,为首次从该植物中分得。

AIM To study the structural characterization of an acidic polysaccharide, H3, isolated from the seeds of Cuscuta chinensis Lam. METHODS Both chemical (such as sugar component analysis, methylation analysis, reduction of uronic acid, partial acidic hydrolysis, etc.) and spectral analysis ( 1HNMR , 13 CNMR , IR, etc.) were used to investigate the structural characterization of H3. RESULTS The molecular weight of H3 was estimated to be more than 1 0×10 6. The analytical results of sugar components indicated that H3 composed of Rha, Ara, Gal, and Gal A. Methylation analysis, partial acidic hydrolysis and 1H , 13 CNMR further identified the linkages and sequence of the residues of H3. CONCLUSION These results suggest that H3 is a highly branched heteropolysaccharide with structure elucidation for the first time.