红花总黄色素体外抑制血小板激活因子受体结合作用的研究

Inhibition of safflor yellow against the binding of platelet activating factor to its receptor in vitro

目的 观察硅胶吸附法制得的红花总黄色素体外对氚标记血小板激活因子 (3HPAF)血小板受体结合的影响 ,试图证明该药为一新型血小板激活因子 (PAF)受体拮抗剂。方法 以洗涤的家兔血小板 (WRP) ,血小板膜 (PM)及血小板膜蛋白 (PMP)为受体制剂 ,配体为3HPAF进行受体结合实验 ,观察红花总黄色素对3HPAF受体结合的抑制作用。结果 3种浓度3HPAF作为配体进行WRP受体结合实验 ,不同浓度的红花总黄色素抑制3HPAF与受体的特异性结合均可见明显的量效关系 ;3种浓度3HPAF作为配体进行PM受体结合实验 ,不同浓度的红花总黄色素抑制3HPAF与受体的特异性结合均可见明显的量效关系 ;两种浓度3HPAF作为配体进行PMP受体结合实验 ,不同浓度的红花总黄色素抑制3HPAF与受体的特异性结合均可见明显的量效关系。

OBJECTIVE In order to clarify whether the Safflor Yellow (SY) is a new platelet activating factor antagonist,we adopted the radio binding assay of SY prepared through silica adsorption procedure.METHODS Three receptor preparations including washed rabbit platelet (WRP),rabbit platelet membrane (PM) and rabbit platelet membrane protein (PMP) extraction were used to observe the inhibition effect of SY against 3HPAF binding to the above receptors individually.RESULTS In WRP radio binding assay of three different 3HPAF concentrations,each specific binding inhibition effect of SY were dosage dependent.In PM radio binding assay of three different 3HPAF concentations,each specific binding inhibition effect of SY were dosage dependent.In PMP radio binding assay of two different 3HPAF concentrations,each specific binding inhibition effect of SY were dosage depenent also.CONCLUSION SY is a novel PAF receptor antagonist.