摘要
目的 研究复方鳖甲软肝片中芍药苷的定量方法。方法 供试品用 5 0 %乙醇冷浸 ,提取液点于硅胶G CMC薄层板上 ,以氯仿 甲醇 冰醋酸 (85∶13∶2 )为展开剂展开 ,晾干 ,喷 5 %香草醛硫酸溶液 ,在 10 5℃烘至斑点清晰 ,用玻璃板盖住 ,周围用胶布密封 ,用CS - 930型薄层扫描仪在λS=5 75nm ,λR=70 0nm下扫描。结果 本方法的平均回收率为 97.6 0 % ,RSD为 3.6 7%。结论 结果显示该方法灵敏、准确、专属性强 ,重现性好 。
OBJECTIVE: To establish a method for the determination of paeoniflorin in Fufang Biejia Ruangan tablet. METHODS: The tablets were extracted with 50% ethanol. The sample solution was applied on a silica gel G-CMC plate and developed with chloroform-methanolglacial acetic acid (85:13:2) and was sprayed with sulfuric acid containing 5% vanillin. The separated spot was determined by scanning with CS - 930 at λS = 575 nm, λR = 700 nm. RESULTS: The average recovery was 97.60% and the RSD was 3.67%. CONCLUSION: The results showed that this method is sensitive, specific, accurate and reproducible.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2001年第3期196-198,共3页
Chinese Pharmaceutical Journal
