去甲二氢愈创木酸与氨甲喋呤、长春新碱配伍对人恶性胶质瘤细胞株的作用

Effects of nordihydroguaiaretic acid treatment combined with Methotrexate or Vincristine on a human malignant glioma cell line

目的 观察体外应用去甲二氢愈创木酸 (NDGA)、氨甲喋呤 (MTX)、长春新碱 (VCR) 3种药物单用或合用对人恶性胶质瘤细胞株SHG 44细胞的作用 ,并探讨其作用的可能机制。方法 用MTT法检测药物作用效应 ,用免疫组化染色法检测细胞CyclinD1基因的蛋白表达情况。结果 ① 3种药物单用及两药合用时随着药物浓度的增加其抗肿瘤效应也增加 ,且两药合用时药物的效应增强 ;②先给NDGA 2 4h后再给MTX或VCR ,与同时给药对该细胞的抗肿瘤效应差异无显著性 (P >0 .0 5 ) ,而先给MTX或VCR2 4h后再给NDGA ,与同时给药对细胞的抗肿瘤效应差异有显著性 (P <0 .0 5 ) ;③免疫组化染色结果显示 ,与对照组相比 ,NDGA处理后该细胞CyclinD1基因的蛋白表达明显降低。结论 NDGA与MTX或VCR间有协同作用 。

Objective To observe the effect of nordihydroguaiaretic acid treatment combined with Methotrexate or Vincristine on a human malignant glioma cell line. Methods The effect of drugs on the cells was measured by MTT, and the protein expression of cyclin D1 gene in the cells was measured by immunohistochemistry assay. Results ①The anti tumor effects of drugs on the cells were increased with the increasing of concentrations, and had a combined function between NDGA and MTX or VCR. ②There was no difference between the effect of treating NDGA with MTX or VCR given at the same time and MTX or VCR given after 24 h of NDGA treatment, but the effects were apparently different when NDGA was administrated after MTX or VCR treatment for 24 h. ③The protein expression of cyclin D1 gene in the cells was apparently reduced after NDGA treatment. Conclusion There is a cooperative function between NDGA and MTX or VCR on the cells, and the function is probably related to the NDGA induced down regulation of protein expression of cyclin D1 gene in the cells.