电离辐射对小鼠腹腔巨噬细胞阴离子通道活动的影响及康复新对其的作用

Effects of irradiation and W_(11)-a_(12) on anion-selective channel of mouse peritoneal macrophage

目的 探讨电离辐射对小鼠腹腔巨噬细胞阴离子通道活动的影响及康复新对其的作用。方法 采用电生理膜片钳离子单通道记录方法。结果 用调理的zymosan刺激腹腔巨噬细胞后 ,阴离子通道开放的阳性率在受照射组显著减少 ,且受刺激至通道开放的时间间隔显著延长。受照射组较正常对照组通道开放概率显著降低、开放时间常数缩短而关闭时间常数延长 ,康复新对正常对照组离子通道活动的影响不显著 ,但可使照射组的通道开放有一定程度的加强。结论 电离辐射抑制巨噬细胞膜上阴离子通道的开放可能是其抑制巨噬细胞功能的一个重要途径。

Objective To study the effects of irradiation and W 11 a 12 ,a kind of repair promoting drug,on anion selective channel in membranes of mouse peritoneal macrophage. Methods The activity of anion selective channel was recorded from cell attached patches with patch clamp techniques. Results The effects of irradiation on anion selective channel in membranes of peritoneal macrophage included:①decreasing the mean number of activated channels by the presence of zymosan; ②prolonging the mean time from stimulus to the opening of channels; ③depressing the opening of channels by decreasing open state probability,shortening open time and prolonging close time. The effects of irradiation could partly be depressed by W 11 a 12 . Conclusion Irradiation will depress the anion selective channel of peritoneal macrophage, which may be an important way to depress the function of macrophage.