CTLA-4Ig预防小鼠自身免疫性肝炎的实验研究

Experimental study of cytotoxic T lymphocyte associated molecule-4Ig for prevention of mice autoimmune hepatitis

目的 研究CTLA 4Ig在防治C5 7BL/6小鼠自身免疫性肝炎上的作用。方法 通过用C5 7BL/6近交系小鼠的肝特异性抗原与弗氏完全佐剂混合物免疫攻击小鼠 ,同时用CTLA 4Ig治疗 ,观察小鼠的肝脏组织学改变。结果 随着免疫次数的增多 ,治疗组结果与正常对照组相似 ;而病理模型组发现炎细胞的浸润 ,肝细胞的肿胀、灶性坏死甚至广泛坏死 ,这与正常对照组有明显差异。结论 CTLA 4Ig能有效地预防C5

Objective To investigate the role of cytotoxic T lymphocyte associated molecule 4Ig(CTLA 4Ig) in the prevention of C57BL/6 mice autoimmune hepatitis. Methods The C57BL/6 mice were intraperitoneally immunized with C57BL/6 mice liver specific protein in complete Freund's adjuvant. At the same time CTLA 4Ig were given to observe the pathologic alteration of C57BL/6 mice liver. Results With the increase of time of immunization, the results in the treatment group were similar to those of the control group; but inflammatory cell infiltration, hepatic cell swelling, focal necrosis and severe hepatocyte damage were found in the pathologic model group. There was a significant difference between the pathologic model group and control one. Conclusion Autoimmune hepatitis of C57BL/6 mice can be effectively prevented by CTLA 4Ig.