豚鼠微血管通透性变化在内耳缺血性损伤中的作用

Role of cochlea microvasculature permeability changes in guinea pigs after inner ear ischemia

目的 研究豚鼠耳蜗微循环障碍动物模型的耳蜗微血管通透性变化特点 ,了解耳蜗微血管通透性变化在内耳缺血性损伤中的可能作用。方法 ①光化学法建立豚鼠耳蜗微循障碍动物模型 ;②改良伊文思蓝荧光法定量研究其耳蜗微血管通透性变化特点 ;③记录CAPN1阈值研究其听力损伤。结果 豚鼠耳蜗发生微循环障碍后 2h和 4h ,伊文思蓝通过耳蜗微血管的渗出量分别为 ( 1 .70 9± 0 .76 9) μg/只和 ( 2 .849± 0 .6 5 3) μg/只 (P <0 .0 1 ) ;CAPN1阈值分别增加 ( 2 4.44± 7.2 7)dBpeSPL和 ( 38.33± 7.91 )dBpe SPL(P <0 .0 1 )。结论 豚鼠耳蜗发生微循环障碍后其耳蜗微血管通透性明显增高并随时间的延长而加重 。

Objective To study the cochlea microvasculature permeability changes of the inner ear in guinea pigs, so as to clarify the possible role of the cochlea microvasculature permeability changes in the ischemic injury of inner ears. Methods Modified method of Evans blue fluorescence was used to observe the changes of permeability of the cochlea microvasculature in the animal model of cochlea microcirculatory disorders which was caused by photochemical reaction. CAPN1 threshold was recorded by using Madsen 2 250 to study the hearing loss. Results The amounts of Evens blue crossing the cochlea microvasculature were (1.709±0.769) and (2.849 ±0.653) μg/per animal 2 and 4 h after the development of cochlea microcirculatory disorder in guinea pigs, respectively ( P <0.01); and their hearing loss were (24.44 ±7.27) and (38.33±7.91)dBpeSPL in 2 and 4 h, respectively ( P <0.01). Conclusion The permeability of the cochlea microvasculature increases along with the duration of cochlea microcirculatory disorder occur and the increase of cochlea microvasculature permeability might be one of the important mechanisms inducing cochlear ischemic lesions.