去氧肾上腺素对肝细胞内游离钙分布的影响

Effects of phenylephedrine on free calcium distribution in hepatocytes

目的 探讨去氧肾上腺素对肝细胞内游离钙分布的影响。方法 应用激光扫描共聚焦显微镜观察去氧肾上腺素单独或在酚妥拉明预处理后对肝细胞作用所引起的细胞内荧光强度的变化。结果 去氧肾上腺素对肝细胞作用后可引起细胞内荧光强度的迅速增强。而在酚妥拉明预处理肝细胞后细胞内荧光强度的变化不显著。同时肝细胞内荧光强度的不均匀分布亦表明游离钙的分布存在着亚分区现象。结论 去氧肾上腺素可通过α受体介导肝细胞内钙信号的变化 。

Objective To investigate the changes of free calcium distribution in hepatocytes after administration of phenylephedrine. Methods The changes of fluorescence intensity were observed by confocal laser scanning microscopy after administration of phenylephedrine alone or pretreated with phentolamine before phenylephedrine administration. Results The fluorescence intensity increased rapidly after administration of phenylephedrine to hepatocytes. When liver cells were pretreated by phentolamine before phenylephedrine administration, the changes of fluorescence intensity not obvious. Meanwhile, the inconformity of the fluorescence intensity in hepatocytes suggested the existence of the second subarea of free calcium distribution. Conclusion Ca 2+ signal can be arisen by phenylephedrine via the α receptor in hepatocytes in vitro . The distribution and dynamic changes of free calcium in hepatocytes display some characteristics.