大肠癌中抑癌基因p33/ING1 mRNA的表达及意义

Expression of tumor suppressor gene-p33/ING1 in colorectal carcinoma

目的 了解p33/ING1表达与大肠癌临床病理特征的关系及其可能作用机制。方法 采用逆转录PCR检测 5 2例散发性大肠癌癌组织及正常大肠粘膜中ING1的表达。结果 在大肠癌组织中p33/ING1mRNA表达较正常粘膜明显减弱 ( 30 /5 2 ,5 7.7% ) ,p33/ING1的低表达与肿瘤的Duke’s分期及淋巴结转移显著相关。

Objective To explore the correlation between the expression of p33/ING1 and the clinicopathological features in patients with colorectal cancer, and to understand the possible mechanism of p33/ING1 in the oncogenesis of colorectal neoplasms. Methods All samples of normal mucosa and cancer tissues from 52 patients with colorectal cancer were detected for their expression levels of p33/ING1 by RT PCR. Results A significant decrease in p33/ING1 mRNA expression was found in 30 out of 52(57.7%) colorectal cancer tissues. The results also showed that repression of p33/ING1 expression markedly related to both the Duke's stage and metastasis. Conclusion Down regulation of p33/ING1 may play an important role in the oncogenesis and development of colorectal carcinoma.