应用胶体金SEA-DIPSTICK法检测日本血吸虫病血清抗体的研究

STUDY ON THE DETECTION OF SERUM ANTIBODY IN SERA OF PATIENTS WITH SCHISTOSOMIASIS JAPONICA BY COLLOIDAL GOLD SEA-DIPSTICK

目的 建立一种快速、简便、实用的检测日本血吸虫病血清抗体的Dipstick方法。方法 应用胶体金SEA -dip stick法检测日本血吸虫病血清抗体。结果 用该法检测急性血吸虫病血清 2 8人份和慢性血吸虫病血清 2 97人份 ,其阳性检出率分别为 10 0 %和 97 31%。在 5 18例正常人和 5 73例其它 4种寄生虫病人血清中有 0 19%假阳性和 2 4%交叉反应。与Dot-ELISA比较 ,经卡方检验表明两者的敏感性无显著性差异 ,而SEA -dipstick法的特异性较Dot -ELISA强。 结论 胶体金SEA -dipstick法检测日本血吸虫病血清抗体有较高的敏感性和特异性 ,并且方法快速 ,操作简便 ,不需特殊仪器设备 ,稳定性高 ,重复性好 ,具有广泛的应用前景。

Aim To develop an accurate,rapid,simple and applicable assay for the diagmosis of Schistosomiasis japonica.Method A time-saving,reliable colloidal gold SEA-dipstik assay was applied to detect serum antibody of schistosomiasis japonica.Results All of 28 sera from patients with accute schistosomiasis showed positive reaction while 289 of 297(97.31%) sera from chronic schistosomiasis were positive by SEA-dipstick assay.1 of 518(0.19%) sera from normal population gave false positive reaction 14 of 573(2.4%) cases of various other parasitic diseases,including paragonimiasis,clonorchiasis,cysticercosis,and trichinellosis,showed cross reaction.SEA-dispstick assay showed similar sensivity and higher specificity as parallel testing by Dot-LISA (P<005).Conclusion Colloidal gold SEA-dipstick assay is not only rapid and simple,but also sensitive adn specific for the detection of serum antibody of schistosomiasis japonica.It will be a practical immunological assay for the diagnosis of schistosomiasis in the future.