旋毛虫成虫、新生幼虫和肌幼虫抗原的保护性免疫研究

STUDY ON THE PROTECTIVE IMMUNITY ACTIVATED BY ANTIGENS OF ADULT WORMS,NEWBORN LARVAE AND MUSCLE LARVAE OF TRICHINELLA SPRIALIS

目的 比较旋毛虫成虫抗原、新生幼虫抗原和肌幼虫抗原接种小鼠诱导产生的保护性免疫。方法 检查肠道成虫、肌幼虫和血液中嗜酸性细胞数 ;用ELISA测血清中抗旋毛虫肌幼虫IgG抗体滴度。 结果 成虫、新生幼虫和肌幼虫抗原免疫组的成虫减虫率分别为 82 19%、72 31%和 42 88% ;肌幼虫减虫率分别为 6 8 83%、78 19%和 5 1 96 %。血清IgG抗体滴度明显升高 ,成虫、新生幼虫和肌幼虫抗原免疫组的GMRT分别为对照组的 7 46倍、5 2 8倍和 4 92倍。血液嗜酸性粒细胞明显增多。结论 旋毛虫成虫抗原、新生幼虫抗原和肌幼虫抗原均可激发特异性体液免疫和细胞免疫 ,诱导小鼠对攻击感染产生保护性。其中 ,成虫抗原和新生幼虫抗原显示出更好的免疫原性 ,而成虫抗原有可能成为较理想的免疫疫苗来源。

Aim To compare the protective immunity against challenge infection in mice immunized by crude antigen preparations derived from adult worms,newborn larvae and musele larvae of T richinella spiralis.Method Intestinal adult worms,muscle larvae and blood absolute eosinophil level were counted;the level of serum IgG to antigens of T.spiralis muscle larvae was detected by ELISA.Results Adults reduimg rate was 82.19%,72.31% and 42.88% in the adult,newborn larvae and muscle larvae antigen groups respectively.Muscle larvae were reduced 68.83%.78.19% and 51.96% respectively.The serum IgG titer in all immunized groups increased significantly,the GMRT values of adult,newborn larvae and mudscle larval antigen groups were 7.46,5.28and 4.92 times higher than that in control group respectively.Periphery blood absolute eosinophil level enhanced significantly.Conclusion The data demonstrate that all of the adult,newborn larvae and muscle larvae antigens of T.spiralis can activate specific humoral and cellular immunity which induce protection to challenge infection in mice.Among these antigens,adult and newborn larvae antigens show better immunogenicity and it may be possible that adult antigen will provide a potential vaccine for trichinosis.