高效液相色谱用于微囊藻毒肽的分离制备

Separation and Preparation of Toxic Peptide in Microcystin Aeruginosa by Hi9h Performance Liquid Chrbmato9raphy

<正> 微囊藻是有毒藻类之一。微囊藻毒素结构复杂,多为肽类,在药理学性质上具有典型肝毒特征。中科院水生所曾对武昌东湖和安徽巢湖的微囊藻毒素作了初步研究并证明为毒肽。为了对此毒肽作进一步结构分析,本文采用反相高效液相色谱对该毒肽进行了纯化制备,取得了色谱纯单峰样品供小白鼠毒性实验,证明其具有与文献报道相符毒性。 本文还考察了流动相中有机改性剂的比例和离子强度对毒肽容量因子(k~′)的影响,并探讨了毒肽在反相键合固定相上的双保留机理。

The toxic peptide from a crude extract of microcystin aeruginosa was isolated and prepared by high performance liquid(?) chromatography and toxicity of the eluates has been tested. Purity of toxic petide prepared in this manner was indicated by further passage through HPLC column and giving rise to a single peak. The experimental results also show that there exists dual retention mechanism in this process.