脂肪酶在负离子化PET表面的单分子层自组装膜研究

Studies on the Lipase Monolayer Film Self-assembly on the Nagatively Charged Poly(ethylene terephthalate) Substrate

研究了脂肪酶分子在表面负离子化聚对苯二甲酸乙二醇酯 (PET)薄膜表面的分子自组装 ,由 AFM和FTIR-ATR等方法研究脂肪酶单分子层膜的表面结构 ;活力测定表明 :脂肪酶 /PET自组装单分子层膜的酶活力表现率约为 2 .

The PET CO - 2 film was prepared and the lipase was assembled on the surface of the PET CO - 2 substrate. The structure of the surface and activity of lipase/PET monolayer were studied by FTIR ATR and AFM and other methods. As shown in the results, AFM images of lipase/PET monolayer indicated that the surface fluctuation is in the range of nanometer scale and the depth of monolayer is less than 15 nanometers. The performance activity of lipase/PET monolayer self assembly film is above 2.0.