5种贝类脂肪含量及脂肪酸组成研究

A Study on the Contents of Fat and Fatty Acids in Five Shellfishes

用氯仿 甲醇法测定了广州海鲜市场上棕带仙女蛤、波纹巴非蛤、文蛤、栉孔扇贝和园华扇贝等 5种贝类的脂肪含量 ,并用GC MS法测定了它们的脂肪酸组成。 5种贝类鉴定出的脂肪酸都在 99% (质量分数 )以上。它们的脂肪含量都大于 1% (质量分数 ) ,园华扇贝的脂肪含量最高。它们的ω 3多不饱和脂肪酸与ω 6多不饱和脂肪酸含量的比值基本上都大于 2。两种扇贝的廿碳五烯酸 (EPA)和廿二碳六烯酸 (DHA)含量都比较高。分析结果表明 ,园华扇贝不仅脂肪含量高 ,而且EPA与DHA的含量也比较高 。

The contents of fat and fatty acids in Callista erycina (Linnaeus), Paphia (Paratapes) undulata (Born), Meretrix meretrix (Linnaeus) , Chlamys farreri (Jones et Preston) and Patinopecten yessoensis (Jay) were studied. Fat was extracted with Bligh & Dyer (B&D) method. The lipid classes were transesterified with potassium hydroxide in methanol. Fatty acid methyl esters (FAMEs) were assayed with GC MS and polar capillary column (HP INNOWax 30 m×0.25 mm i.d.×0.25 μm). GC injector temperature was 220 ℃. The column temperature was programmed from 150 ℃(1 min) to 200 ℃ at 10 ℃/min and then from 200 ℃ to 250 ℃ at 2 ℃/min. FAMEs were identified by MS library, and part by their standards. Total identified fatty acids were over 99% for all samples. Fat contents of them were all over 1% by wet samples. And ratios between ω 3PUFA and ω 6PUFA were above 2 by and large. Patinopecten yessoensis (Jay) contains more fat and the valuable fatty acids, EPA and DHA. It is suitable to use it as the source of EPA and DHA.