二硫化碳接触工人的血清热应激蛋白70、90抗体滴度及其意义分析

Analysis on the Titer of Serum Antibodies to Heat Stress Protein 70 and 90 in Workers Exposed to Carbon Disulfide

为了解二硫化碳 (CS2 )接触工人的血清热应激蛋白 (HSPs)抗体滴度及探讨HSPs抗体滴度作为CS2 接触的分子生物标记物的可行性。选择了某化纤厂 86名工人 (对照组 2 7名 ,接触组 5 9名 )。用Westernblot-ELISA法对工人血清HSP70和HSP90抗体水平进行了检测。采用logistic回归分析技术 ,分析了HSPs抗体与CS2 接触之间的关系。除HSP90抗体 1∶80滴度外 ,其余滴度接触组工人血清HSPs抗体阳性率明显高于对照组 ,差异有显著性 (P <0 0 5 )。用logistic回归分析控制了混杂因素的作用后 ,这种联系仍然存在。血清HSP70、HSP90抗体滴度可反应CS2 的接触状况。把血清HSPs抗体滴度作为分子生物标记物对接触CS2

In order to investigate the titer of serum antibodies against heat stress protein 70(HSP70)and 90(HSP90)in workers exposed to carbon disulfide (CS 2),and the possibility of taking antibodies to HSPs in serum of workers as biomarkers,serum anti HSP70 and anti HSP90 were detected by Western blot enzyme linked immuno sorbent assay(Western blot ELISA).A total of 86 workers in a viscose rayon factory were selected and divided into 2 groups(27 workers as the control group,and 59 as the exposed group).Logistic regression model was used to analyze the relationship between anti HSP and CS 2 exposure.Results showed that the positive occurrence rates of antibodies to HSP70(1∶10,1∶20,1∶40,1∶80)and HSP90(1∶10,1∶20,1∶40)in exposed group were higher than those in the control group(P<0 05).It could conclude that the titer of serum antibodies to HSP70 and HSP90 had close relationship with the CS 2 exposure.Antibodies to HSPs may potentially serve as a serum biomarker to assess CS 2 exposure.