辐射小鼠骨髓CD34^+细胞的变化及其意义

The changes of CD34^+ cells in C57 mouse bone marrow after irradiation and their roles in dysfunction of hematopoiesis

目的 研究γ射线照射后C5 7小鼠骨髓中CD34+细胞的数量变化规律及其意义。方法 流式细胞仪测定CD34+细胞在骨髓有核细胞中的比例 ;AnnexinV FITC试剂盒检测骨髓细胞的凋亡 ;细胞固定后PI染色测定细胞周期。结果 ①CD34+细胞在骨髓有核细胞中的比例随照射剂量的加大而降低 ,在 5 5Gy照射后 14d内小鼠CD34+细胞的减少表现为持续性 ;②小鼠照射后6h骨髓细胞凋亡率最高 ,以 5 5Gy照射组最为明显 ;③ 5 5Gy照射后小鼠骨髓细胞周期紊乱。 结论 γ射线损伤骨髓中的干祖细胞 ,造成骨髓中干祖细胞的数量减少 ,其途径之一是诱导骨髓细胞凋亡。

Objective\ To observe the changes of CD34 + cells in C57 mouse bone marrow after irradiation and investigate the role of apoptosis in radiation\|induced dysfunction of hematopoiesis. Methods Flow cytometric enumeration of CD34 + hematopietic stem and progenitor cells by double fluorescent labeling apoptosis detection by Annexin V\|FITC kit,and cell cycle detection by PI labeling were carried out. Results\ ①Compared with the normal group,the percentage of CD34 + cells in bone marrow nucleated cells decreased at least for 14 days after irradiation,and the changes were related with irradiation doses.②At 6 h after irradiation,the largest amount of apoptopic cells could be detected.③Bone marrow cell cycle was perturbed after 5 5 Gy irradiation. Conclusion\ The percentage of CD34\++ hematopietic stem and progenitor cells in C57 mouse bone marrow decreased after irradiation,and apoptosis might be responsible for the changes of the bone marrow cells.\;