人参皂甙清除反应性氧代谢产物预防大鼠膜性肾病

Role of reactive oxygen metabolites (ROM)in cationized bovine serum albumin (C-BSA)induced membranous nephropathy (MN) inrats and the protective effect of Chineseherb extract

本试验采用阳离子化牛血清白蛋白（Ｃ－ＢＳＡ）制作大鼠膜性肾病模型。大鼠随机分四组，除正常对照外，其余三组在往射Ｃ－ＢＳＡ的同时，分别注射等渗盐水、去铁敏（ＤＦＯ）和人参皂甙，共５周。结果ＤＦＯ和人参皂甙能显著降低蛋白尿，降低血清及肾皮质中脂质过氧化终产物丙二醛（ＭＤＡ）含量，提高血清及肾皮质中超氧化物歧化酶（ＳＯＤ）和谷胱甘肽过氧化物酶（ＧＳＨ－ｐｘ）活性，组织学检查显示ＤＦＯ和人参皂甙轻度改善ＩｇＧ，Ｃ３和ＢＳＡ沉积，显著改善上皮细胞肿胀和足突融合。提示中药提取物人参皂甙与羟自由基清除剂ＤＦＯ相似，能抗脂质过氧化，保护抗氧化酶活性，降低Ｃ－ＢＳＡ大鼠蛋白尿。

he purpose of this experiment was tostudy the role of ROM in C-BSA-inducedMN of rats and the protective effect of Gin-senoside(GS) . 31 Wistar rats were random-ly assigned to 4 groups ,each preimmunizedsubcutaneously with mg C-BSA emulsifiedin incomplete Freund's adjuvant. Seven dayslater, eontrol group received saline only,other groups received intraveneous C-BSA3. 5 mg every other day until the end of the4th week. Saline, DFO and GS were givenseperately in different groups before the in-jection of C-BSA and continued during anti-gen challenge. DFO and GS treatment re-sulted in significant decrement of protein-nuria compared with control. DFO and GSalso resulted in significant decrement ofMDA content (P<0. 01 ) and increment ofSOD and GSH-px activities (P < 0. 01 inserum and renal cortex) as compared withthe control. If study showed that DFO andGS slightly attenuated CBSA rats glomeru-lar IgG,C3 or BSA deposition. LM showedthickening and spikes of GBM in experimen-tal model but a mild extent in DFO and GS-treated rats. EM showed DFO and GS coulddecrease subepithelial electron-dense de-posits and attenuate fusion of foot process.Our results suggested that ROM might me-diate in part glomerular injury in C-BSA-in-duced MN. GS could reduce the extent oflipid peroxidative injury , protect the activityof lipid peroxidative injury, protect the ac-tivity of anti-oxidase and recuce proteinuri-a .