摘要
利用限制性内切酶酶切和分子杂交等手段,对弗兰克氏菌菌株At4和Hr16的固氮基因克隆的nifH(pPC1201)、nifD(pPC1202)及nifK(pPC1203)基因为探针杂交,定位了At4和Hr16的nifH和nifD基因。同时发现,一条1.3kb的BamHI片段丢失。
Nif gene clones from Fankia strain At4 and Hrl6 were analysed physically using digestion of restriction endonucleases, molecular hybridization and other techniques. And then, they were hybridized with nifH (pPC1201), nifD (pPC1202) and nifK (pPC1203) genes from Klebsiella pneumoniae, respectively. At the same time a loss of 1.3-kb BamHI DNA fragment was discovered.
出处
《西北农业大学学报》
CSCD
1992年第1期5-10,共6页
Journal of Northwest Sci-Tech University of Agriculture and Forestry（Natural Science Edition)
基金
国家自然科学基金
关键词
弗兰克氏菌
固氮基因
基因定位
Frankia, molecular hybridization, Klebsiella pneumoniae, nitrogen fixation genes, gene mapping, transposon, sub梒lone
