大蒜病毒病原的鉴定及组培脱毒研究

On Identification and Virus-free Meristem Culture of Garlic Virus Diseases

系统鉴定的结果表明,为害大蒜的病毒有GLV,GMV和TMV。GMV引起大蒜花叶及条纹花叶,而GLV在大蒜上表现潜隐症状。GLV的紫外吸收峰最低在245.2nm,最高在275.7nm,其A260/280为1.36。GLV和GMV粒子大小分别为548～700×13nm和750～773×13nm。德国GLV抗血清仅与GLV有阳性反应。采用0.10～0.25mm生长点能完全脱除病毒,而用0.30～0.70mm有部分脱毒效果。

The results from systematical identification showed that the viruses infecting garlic include garlic latent virus (GLV), garlic mosaic virus (GMV) and tobacco mosaic virus (TMV). GMV causes common mosaic and stripe mosaic in leaves: GLV showed latent symptoms in the infected garlic. The purified preparation of GLV showed that the ultra-voilet absorption was 245.2 nm at minimum and 257.7 nm at maximum whose A260 / 280 was 1.36. Particles of GLV and GMV were 548-700 x 13 nm and 750 - 773 x 13 nm. Germany GLV antiserum is related only to GLV. According to virus-free meristem cultural experiment, virus-free garlics can be completely successful using 0.10-0.25 mm long meristems but partially by 0.30-0.70 mm long meristems.