加热霍乱类毒素的制备及其抗原性的研究

STUDY ON PREPARATION AND ANTIGENICITY OF A HEATED CRUDE CHOLERA TOXION

粗制霍乱肠毒素经60℃水浴,5分钟后其毒性丧失99％,抗原结合性保留87.5％。PAGE显示:毒素已成为大分子聚合物。将这种聚合物经口免疫小鼠,产生良好的肠道抗毒免疫和一定程度的血清抗体反应。

Crude cholera toxin, heated with water bath at 60℃ for 5 min, Lost 99 per cent of toxicity and 12.5 per cent of antigenicity. PAGE revealed that treated toxin had aggregated to polymer. P. O. immunized mice can produce a good intestinal antitoxinal protection and a certain amount of serous antibodies.