聚合酶链反应在检测乙肝病毒感染中的应用

APPLICATION OF POLYMERASE CHAIN REACTION IN DETECTING HEPATITIS B VIRSUS INFECTION

应用ＰＣＲ技术对医院临床实验室检测的２６５例乙肝患者血清的ＥＬＩＳＡ五项结果所用血清作了复检，结果发现：ＥＬＩＳＡ五项指标全阴４０例，单纯ＨＢｓＡｂ阳性４０例，单纯ＨＢｃＡｂ阳性４０例，ＨＢｓＡｇ、ＨＢｃＡｂ两项阳性４０例，ＨＢｓＡｇ、ＨＢｅＡｂ和ＨＢｃＡｂ三项阳性４０例，ＨＢｓＡｇ、ＨＢｅＡｇ和ＨＢｃＡｂ三项阳性４０例，ＨＢｓＡｇ和ＨＢｅＡｇ均阳性１５例及单纯ＨＢｓＡｇ阳性１０例，而ＰＣＲ检测Ｈ３Ｖ－ＤＮＡ的阳性例数分别为２，３，７，１６，１１，３８，１５和３例。ＥＬＩＳＡ法和ＰＣＲ法检测的目的物本不相同，其临床意义，学者对ＥＬＩＳＡ法已有不少报道，ＰＣＲ法检出ＨＢＶ－ＤＮＡ证明血清中有乙肝病毒存在。对ＰＣＲ检测结果，结合ＥＬＩＳＡ检测结果作了讨论。

Two-and-half pair parameters of 265 sera of HB patients from three hospitals in Chengdu for HBV infection detected by same ELISA reagent kit were re-detected using PCR technique and the results revealed that 2 sera were positive in PCR from 40sera which all were negative in ELISA,3 sera positive from 40 sera being HBsAb positive only,7 sera positive from 40 sera being HBcAb positive,11 sera positive from 40 sera being HBsAg,HBeAb and HBcAb positive,38 sera positive from 40 sera being HBsAg,HBeAg and HBcAb positive, 15 sera all positive from 15 sera being HBsAg and HBeAg positive,and 3 sera positive from 10 sera being HBsAg positive respectively.The substances to be detected are different in ELISA and PCR and the chinical significance for ELISA has been reported elsewhere while that for PCR proves the presence of HBV in the serum tested. The results of PCR are discussed in terms of that of ELISA.