人乳头瘤病毒DNA的限制性核酸内切酶分析

RESTRICTION ENZYME ANALYSIS OF HUMAN PAPILLOMA VIRUS(HPV)DNA FROM COMMON WARTS

用差速离心和CsCl平衡密度梯度离心法,从收集的寻常疣中分离并纯化了人乳头瘤病毒(HPV),从中抽提病毒DNA,经四种限制性核酸内切酶消化后,其消化产物进行琼脂糖凝胶电泳。结果显示EcoRI,BamHI,HindⅡ,HindⅢ分别将环形的HPV DNA裂解成1、2、3、2个片段,同时以λDNA的HindⅢ片段为标准核酸计算了各HPV DNA片段的分子量。

After HPV DNA from purified common warts HPV was digested by restriction endonucleases、EcoR Ⅰ,BamH Ⅰ,Hind Ⅱ,Hind Ⅲ,the digested products were analysed by electrophoresis on the agarose gel. The results showed that EcoR Ⅰ,BamH Ⅰ,Hind B Ⅱ,Hind Ⅲ cleavaged the ds circular DNA into 1,2,3,2 fregments respectively.Molecular weights of the fregments were estimented by using the λDNA—Hind Ⅲ fregments as standard nucleic acid.