蜀柏毒蛾核型多角体病毒（NPV）DNA的初步研究

Preliminary Study on the Nucler Polyliedrosis DNA of Parocneria orienta Chao

经ＳＤＳ—冷酚法由纯化的蜀柏毒蛾ＮＰＶ颗粒抽提到了ＮＰＶ的ＤＮＡ．经ＳｅｐｈａｄｅｘＧ—１００柱层析和聚丙烯酰胺凝胶电泳鉴定，该ＤＮＡ是均一制剂．ＰｏＮＰＶ－ＤＮＡ的Ｔｍ值测定为７０℃，经限制性内切酶ＨｉｎｄⅢ和ＥｃｏＲⅠ酶切，琼脂糖电泳分析测得该ＤＮＡ分子量约为５５×１０６道尔顿左右．

The DNA had been extracted from the purified PoNPV particles by SDS-cold phenol method,and identified as a homogenious preparation either by Sephadex G-100 chromatography or by polyacrylamide gel electrophoresis. An approximate estimated Mol. Wt of 55 ×106 dalton was determined by restrictive enzyme digestion (EcoRI,HindⅡ) and agarose gel electrophoresis. Its Tm value was about 70℃.