鸡传染性法氏囊病病毒适应于Vero细胞的初步研究

The Preliminary Study of Infectious Bursal Disease Virus Adapted to Vero cell Line

将国内６个省市（广东、巾东、河北、辽宁、、新疆和北京）的７株鸡传染性法氏囊病（ＩＢＤ）的法氏囊组织处理后，直接在Ｖｅｒｏ细胞上盲传３～４代，均能不同程度的产生特征性细胞病变效应（ＣＰＥ），并通过选用具有广谱性的抗法氏囊病病毒的单克隆抗体（ＩＢＩ）Ｖ－ＭｃＡｂ）采用间接免疫荧光（ＩＦＡ）和碱性磷酸酶—抗碱性磷酸酶桥联酶显色技术（ＡＰＡＡＰ）的方法对７株分离毒的Ｖｅｒｏ第８代细胞进行鉴定，又用逆转—聚合酶链式反应（ＲＴ－ＰＣＲ）对７株分离毒的第１４代Ｖｅｒｏ细胞毒进行进一步证实，结果表明这７株ＩＢＤ囊毒均适应于Ｖｅｒｏ细胞上，这是国内首次报道将组织毒直接适应于Ｖｅｒｏ传代细胞上。

After treatment 7 field isolates of infectious bursal disease(IBD) virus from six provinces inChina blindly passaged for three to four generation on Vero cell Iine,all of them could produce the CPE,Group specific anti infectious bursal disese virus(IBDV)monoclonal antibodies were chosen to detect Vero cell culture adapted isolates of IBDV at the 8th passage by indirect immunofluorescent assay(IFA ) and alkalinephosphatase antialkaline phosphatase complx bridge(APAAP)techniques,and the cell culture adapted iso-Iates at 1 4th the passage were further comfirmed by reverse transcription polymerase chain reaction (RT PCR).The results shwed that the tissue viruses of 7 field isolates all had adapted to the Vero cell Iine,this isfirst reported in China.