中国对虾幼体和仔虾消化酶活力及氨基酸组成的研究

STUDIES ON DIGESTIVE ENZYMES AND AMIND ACID OF LARVAL AND POST LARVAL STAGES OF PRAWN PENAEUS CHINENSIS (O'SBECK 1965)

于1987年5月初在山东日照市涛雒对虾育苗场采集中国对虾虾苗;成虾于1986年春、秋两季取自青岛胶州湾水域的网捕成虾。以酶学分析方法作试验。结果表明,1.虾苗酶活力结果为:类胰蛋白酶及胃蛋白酶的活性有差异,溞状幼体期<糠虾幼体期<仔虾期;酶活性随着生长发育而增高,类咦蛋白酶活性比胃蛋白酶高4倍左右。淀粉酶的活性是溞状幼体期>糠虾幼体期>仔虾期;纤维素酶活力极微;淀粉酶活性是纤维素酶的6—13倍左右。育苗期几种消化酶的活性均远远低于成虾。2,育苗期幼虾的氨基酸含量,随着生长发育而增高,在各期,氨基酸总和的差别是:溞状幼体期<糠虾幼体期<仔虾期。

The larvae of prawn Penaeus chinensis were collected from Shrimp Farm,.Taoluo, Rizhao, in May, 1987. The adult prawns were collected from Jiaozhou Bay, Qingdao during spring and autumn, 1986. This experiment was conducted with the enzyme analytical method. The results indicate that:1. The activities of trypsin-like enzyme and pepsin were different in different stage of the larvae of prawn: Penaeus chinensis Zoea stage< Mysis stage< Post larval stage.The activities of trypsin-like enzyme and pepsin increased with growth. Activity of trypsin-like enzyme was about 4 times higher than that of pepsin.2. The activity of amylase in different stage was Zoea stage>Mysis stage>Post larval stage. The activity of cellulase was fairly low. Activity of amylase was 6 to 13 times higher than that of cellulase. The activity of digestive enzyme in the larvae of prawn was lower than that in the adult prawn.3. The contents of amino acid in the larvae of prawn increased with growth. The contents, of amino acid in different stage are zoea stage<Mysis stage<Post larval stage.