马铃薯提取液中生理活性物质的研究 Ⅱ马铃薯提取液中的内源激素对植物愈伤组织生长和分化的影响

Studies on Physiological Active Substances in the Potato Extract ⅡEffects of Endogenous Hormones in Potato Extract oh the Growth and Differentiation of the Plant Callus

本文证实了马铃薯提取液中存在IAA和ABA.实验结果指出,在马铃薯提取液中ABA的含量比其他内源激素要高.ABA对植物愈伤组织的生长和分化可能起着主导作用.由于马铃薯的品种,发育状况和块茎不同部位的差异,马铃薯提取液对植物愈伤组织的生长和分化的效应也随之而异.根据上述情况,必须选择适合的马铃薯块茎作材料,同时在组织培养中应用马铃薯提取液时尚需适当添加NAA和KT.

In this paper, we indicated that IAA and ABA exist in the potato extract. The results of the experiment showed that ABA content was higher than the other endogenous hormones in potato extract. It was suggested that, of endogenous hormones in potato extract, ABA might be a main hormone affecting the growth and differentiation of the plant callus. There were evident differences in the effects of potato extracts on the growth and differentiation of plant callus due to different potato materials including cultivars, development stage and parts of tuber. From the above, we conclude that selecting better material of potato tuber and supplementing suitable NAA and KT are necessary for the application of potato extract in plant tissue culture.