HLA-DQ等位基因与哮喘相关性研究

Association between asthma and the polymorphism of HLA-DQ genes

目的 探讨在中国汉族哮喘家系中 ,HLA DQ基因与哮喘的相关性。方法 对 98例哮喘家系成员 ,用PCR 序列特异性引物 (PCR SSP)技术对HLA DQA1和B1进行基因分型 ,并与正常对照进行比较。结果 发现DQA1 0 10 1和DQA1 0 6 0 1等位基因频率在哮喘患者组 (4 0 0 % ,45 0 % )较正常对照组 (16 4% ,13 4% )显著升高 (χ2 =6 186 0 ,P <0 0 5 ,RR =3 39;χ2 =11 6 0 90 ,P <0 0 1,RR =5 2 7) ;DQB1 0 30 3和DQB1 0 6 0 1等位基因频率在哮喘患者组 (5 5 0 % ,47 5 % )较正常对照组 (13 7% ,13 7% )显著升高 (χ2 =15 740 0 ,P <0 0 1,RR =7 6 8;χ2 =10 930 0 ,P <0 0 1,RR =5 6 9)。同时发现HLA DQB1 0 2 0 1等位基因频率在对屋尘螨抗原特异性IgE反应哮喘家系成员 (39 4% )较家系中非特应症者 (12 0 % )显著高频表达 (t =2 382 5 ,P <0 0 5 )。结论 HLA DQA1 0 10 1, 0 6 0 1和DQB1 0 30 3, 0 6 0 1是哮喘遗传易感等位基因 ;HLA DQB1 0 2 0

Objective To investigate association between asthma and HLA DQ genes in Chinese asthma family. Methods HLA genotyping was done for HLA DQ using polymerase chain reaction sequence specific primers(PCR SSP) in 98 members of 32 Chinese asthma families and 67 normal controls. Results The allele frequencies of DQA1 *0101 and DQA1 *0601 in asthmatics (40 0% and 45 0% respectively) were significantly higher than those in normal controls (16 4% and 13 4% respectively; χ 2=6 186 0, P <0 05, RR =3 39 and χ 2=11 609 0, P <0 01, RR =5 27 respectively) The allele frequencies of DQB1 *0303 and DQB1 *0601 in asthmatics (55 0% and 47 5% respectively) were also significantly higher than those in normal controls (13 7% and 13 7% respectively; χ 2=15 740 0, P <0 01, RR =7 68 and χ 2=10 930 0, P <0 01, RR =5 69 respectively) The allele frequency of DQ *0201 (39.4%) was significantly higher in members with positive specific IgE response to dermatophagoides than that in non atopy members (12.0%) in asthma family ( t = 2.382 5, P <0.05). Conclusion HLA DQA1 *0101, DQA1 *0601 and HLA DQB1 *0303, DQB1 *0601 may be the susceptible alleles of asthma HLA DQB1 *0201 allele may limit the specific IgE response to dermatophagoides