针刺与中药复元合剂的益智实验研究

Experimental Studies of Acupuncture and Fuyuan Mixture in Improving Intelligence

【目的】进一步观察针刺与中药对记忆功能的作用。【方法】将 12 9只NIH雄性小鼠随机分 6组进行动物实验 ,用东莨菪碱造成记忆获得障碍性模型 ,采取跳台法、复杂迷宫趋食法分别观察了针灸 (取穴百会穴、大椎、神庭、足三里等 )、中药复元合剂 (由鹿茸、人参、黄精、枸杞子、益智仁、丹参等组成 )对模型小鼠的学习记忆功能的改善作用 ,并以西药尼莫地平作为对照。【结果】跳台法实验结果显示 ,针灸组与中药针灸组均可使记忆障碍模型鼠在造模 2 0min、 2 4h后跳台反应的潜伏期延长 ,触电时间缩短 ,错误次数减少 (与模型组比较 ,P <0 .0 5或P <0 .0 1) ;复杂迷宫趋食法的实验结果显示 ,针灸组与中药组均可显著缩短模型鼠到达迷宫终点的时间 (与模型组比较 ,P <0 .0 5或P <0 .0 1)。【结论】针灸、补肾益脑中药复元合剂对东莨菪碱致记忆障碍均有改善作用 。

Objective To further explore the effects of acupuncture and Fuyuan Mixture(FM) on learning and memory. Methods One hundred and twenty_nine NIH male mice were allocated to six groups:Group A (normal control group),Group B(model group),Group C(treated with acupuncture),Group D(treated with FM),Group E(FM and acupuncture),and Group F(treated with nimodipine). Model mice with acquired memory disorder were induced by scopolamine.Baihui,Dazhui,Shenting and Zusanli were chosen as the acupoints.FM is composed of Cornu Cervi Pantotrichum,Radix Ginseng,Rhizoma Polygonati,Fructus Lycii,Fructus Alpiniae Oxyphyllae,Radix Salviae Miltiorrhizae,etc..The methods of platform jumping and finding food in complex maze were used to observe their learning and memory. Results Compared with Group B,incubation period was prolonged,electric shock time shortened and errors reduced at 20 min and 24h after modeling in Group C and Group E,and time for finding food in complex maze was shortened in Group C and Group D(P< 0.05 or?P< 0.01 ). Conclusion Acupuncture and FM exerts a certain effect in improving intelligence.