连黛片药物血清对人胃癌细胞株MGC803的作用

Effects of Liandai Tablet and Its Main Active Components on Gastric Cancer Cell Strain MGC-803

【目的】探讨比较中药连黛片及其主要单体成分在诱导癌细胞凋亡及引起DNA损伤方面的不同药理作用。【方法】用复方连黛片的大鼠药物血清 ,以及黄连的主要药效成分小檗碱、青黛的主要药效成分靛玉红分别作用于人胃癌细胞株MGC 80 3 ,观察药物对细胞生长和凋亡的影响 ,及其对细胞DNA损伤的作用。【结果】连黛片血清高低剂量组及小檗碱各剂量组细胞与相应对照组细胞生长形态相比较差异较大 ;甲基绿—派诺宁染色后 ,细胞表现出典型的凋亡形态 ;对细胞生长率均有明显抑制。靛玉红各浓度组对细胞生长抑制不明显。琼脂糖凝胶电泳分析表明 :小檗碱各浓度组可使细胞DNA裂成大片段 ;连黛片血清各浓度组对MGC 80 3细胞染色质DNA的损伤作用较小檗碱各浓度组小 ;靛玉红在实验浓度下对细胞的DNA无损伤作用。【结论】中药连黛片大鼠药物血清与小檗碱一样均能抑制癌细胞生长及诱导细胞凋亡 ,但其作用比小檗碱弱 ,对DNA的损伤程度也较轻 。

Objective To compare the pharmacological actions of Liandai Tablet(LT) and its main active components on apoptosis of gastric cancer cells and DNA damage. Methods Effects of Liandai Tablet(LT) and its main active components,berberine and indirubin,on growth and apoptosis of gastric cancer cell strain MGC_803 were explored and their effects on DNA damage were also studied. Results LT serum in high and low dosages and berberine could inhibit the growth of MGC_803 as compared with the control group,and typical morphological features of apoptosis were found in the MGC_803 by methyl green pyronin stain assay.But indirubin at various concentrations showed no obvious inhibitory effects. Agarose gel electrophoresis assay revealed that the MGC_803 cell DNA was split into large fragments when treated with berberine. Conclusion LT serum exerts a similar inhibitory effect on the growth and apoptosis of gastric cancer cells as compared with berberine.The effects of LT at various serum concentrations on MGC_803 DNA was less than that of berberine,and indirubin at the given concentration had no this effect.