高效液相色谱法测定石杉碱甲片的含量及其有关物质

Assay of Content of Huperzine-A Tablets,Tests for Uniformity of Content and Its Related Substances by HPLC

目的 :采用高效液相色谱法测定石杉碱甲片的含量、含量均匀度以及有关物质。方法 :采用ODS -C1 8柱 ,以pH 3 2磷酸三乙胺缓冲液 -水 (5 0∶5 0 )为流动相 ,流速 1mL·min- 1 ,用紫外检测器于 30 7nm波长处检测。结果 :石杉碱甲在浓度为 12 8～ 15 9 3μg·mL- 1 范围内 ,浓度与其峰面积呈良好的线性关系 (r =0 99996 )。平均回收率为 10 0 3% ,RSD =0 44 %(n =2 1)。检出的最低量为石杉碱甲 6ng。结论 :较原试行标准简便 ,准确。

Objective:To establish a HPLC method for the assay of the content of huperzine-A tablets,the uniformity of content and its related substances.Method:Using ODS-C 18 column,the mixture of buffer solution of phosphoric acid-triethylamine(pH 32)and water(50∶50)as mobile phase,at a flow rate of 1 mL·min -1,wavelength of the detector was 307 nm.Results:There is a good linear relationship within the range of 128～15932 μg·mL -1(r=0999 96).The average recovery is 1003%,RSD is 044%(n=21).The lowest limit of detection is 6 ng.Conclusion:The new method is more rapid,simple and accurate than the old one.