摘要
目的 :建立沙美特罗对映体拆分方法。方法 :以α1 -酸性糖蛋白 (α1 -AGP)为固定相 ,考察了流动相pH、有机改性剂及流速对分离的影响。结果 :以 10mmol·L- 1 醋酸钠 (pH 4 0 ) -异丙醇 (98∶2 )为流动相 ,流速为 0 9mL·min- 1 ,在α1 -AGP柱上拆分了沙美特罗。结论 :流动相pH和有机改性剂的量是优化分离带正电荷对映体的分离的重要因素。同时可以在一定的流速范围内通过调节流速来提高柱效、改善分离。
Objective:To develop an enantiomeric separation method of salmeterol by HPLC using α 1-acid glycoprotein (α 1-AGP)chiral stationary phase.Method:Influences of the pH value of the mobile phase,organic modifiers,and flow rate on the enantiometric separation of salmeterol using α 1-AGP chiral stationary phase were investigated.Results:Salmeterol enantiomers were separated on an α 1-AGP chiral stationary phase column with a mobile phase consisted of 10 mmol·L -1 NaAc(pH 40)-isopropanol(98∶2)at a flow rate of 09 mL·min -1.Conclusion:The pH value of the mobile phase and the organic modifier concentration were very important parameters when optimizing the enantiomeric separation of the cationic racemates.Higher column efficiency was obtained with lower flow rates.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2001年第2期97-100,共4页
Chinese Journal of Pharmaceutical Analysis
基金
上海市卫生局资助课题
上海市药检所资助课题!编号 98419