鲫鱼(Carassius auratus)卵和胚胎中母源性免疫球蛋白的定位

Location of Maternal Immunoglobulin in the Egg and Embryo of Crucion Carp (Carassius auratus)

自鲫鱼血清中沉淀出免疫球蛋白 (Ig)并层析纯化 .纯化后的Ig加福氏完全佐剂和福氏不完全佐剂免疫大白兔制得特异抗血清 .免疫组化研究显示母源性Ig最初是在Ⅳ时相早期卵中出现 .在卵子的发育过程中 ,Ig遍布整个卵子并由卵膜至卵中心逐渐减少 ,其中卵膜内壁Ig最多 .在心跳期前的胚胎发育过程中 ,母源性Ig存在于卵膜内壁、胚胎和卵黄中 ,至尾鳍形成期完全消失 ;首先是胚胎和卵黄中的Ig消失(心跳期 ) ,然后是卵膜内壁的IgM消失 (尾鳍形成期 ) .在胚胎发育过程中 ,Ig亦遍布于卵黄之中 ;其中 ,卵膜内壁含Ig最多 ;在胚胎和卵黄中无Ig集中的现象 .

Immunoglobulin (Ig) were precipitated and purified from crucian carp's sera. After immunized with pure Ig of crucian carp in FCA and FIA, we obtained special antisera from rabbit Immunohistochemical studies showed maternal Ig began to appear in the early Ⅳ phase egg During the development of egg, Ig dispersed throughout the yolk of the egg and decreased from the membrane to the center, and more Ig localized inside the internal membrane than at other sites Maternal Ig exist in the internal membrane, embryo body and yolk during embryonic development until heart beating stage and disappeared completely at the stage of caudal fin forming, Ig in embryo and yolk disappeared first, and then disappeared inside the internal membrane of egg During the embryonic development, Ig also dispersed throughout the yolk of the embryo, and more Ig localized inside the internal membrane than at other sites; there was no phenomena of Ig concentrating in the embryo or yolk