pH值对甲肝减毒活疫苗后处理得率的影响

The Effect of pH on Gaining-Rate During Processing Technology of Live Attenuated Hepatitis a Vaccine(H2 Strain)

在甲肝减毒疫苗生产工艺中不同pH对疫苗得率有一定的影响 ,为提高疫苗的产量 ,在传统的氯仿抽提条件下 ,使用不同pH值 (pH1.0～ 12 .0 )的磷酸盐缓冲体系进行氯仿抽提 ,检测抽提后甲肝病毒(HepatitisAVirus ,HAV)抗原滴度和感染性滴度 ,结果表明 :经氯仿抽提后 ,HAV抗原滴度和感染性滴度均在pH8.0～ 9.0时最高 ,相应得率也比常规处理的得率有显著的提高 .说明改进pH抽提条件对甲型肝炎减毒活疫苗后处理工艺效率的提高 ,成品疫苗产量的增加具有实际应用价值 .

The pH plays an important role in processing technology of live attenuated hepatitis A vaccine. In order to increase te productivity of the vaccine, authors selected different pH(1.0～12.0) to study the different gaining-rate of product. The results showed that pH(8.0～9.0) were the best pH range, in this range the gaining-rate of processing thchnology was much higher than that of traditional processing.