成年大鼠睾丸Smad1和Smad5的免疫组织化学研究

Expression of Smad1 and Smad5 Proteins in the Adult Rat Testis

目的 :探讨骨形态形成蛋白的细胞内信号传导分子Smad1和Smad5蛋白在成年大鼠睾丸内的表达。 方法 :应用免疫组织化学ABC法结合葡萄糖氧化酶 DAB 硫酸镍铵增强技术 ,检测成年大鼠睾丸Smad1和Smad5蛋白的表达和定位。 结果 :Smad1蛋白表达于大鼠睾丸精曲小管的各级生精细胞的胞质内 ,呈淡染的紫蓝色颗粒 ,胞核为阴性 ,免疫染色阳性细胞主要包括精原细胞、初级精母细胞、次级精母细胞和精子细胞 ;而Smad5蛋白阳性染色不明显 ,只有睾丸间质细胞呈弱阳性反应 ,免疫反应阳性物质主要定位于细胞质。 结论 :Smad1蛋白主要表达于睾丸精曲小管各级生精细胞 ,Smad5蛋白表达于间质细胞 ,为揭示TGF β超家族在精子发生中的分子机理提供了直接证据。

Objectives : To study the expression and localization of Smad1 and Smad5 proteins, intracellular signaling molecules of bone morphogenetic proteins, in the adult rat testis. Methods: Immunohistochemical ABC method with glucose oxidase DAB nickel enhancement technique was used in the present study. Results: Smad1 immunoreactivity was mainly located in the spermatogonia, spermatocytes, spermatids. The reactive substance distributed in cytoplasma with negative nuclei. In contrast, Smad5 was weakly expressed in cytoplasma of Leydig cells. Conclusions: Smad1 expression in spermatogenic cells and Smad5 expression in Leydig cells provided direct evidence for elucidating the molecular mechanism of TGF β action during spermatogenesis. Ntl J Androl,2001,7(1):10～13