骨巨细胞瘤中p185和p53的免疫组化研究

Immunohistochemical Analysis of p185 and p53 Protein Expression in Giant Cell Tumors of Bone

目的 探讨p185和p53在骨巨细胞瘤 (GCT)的表达及GCT病理分级和复发的关系。方法 应用SP免疫组织化学方法检测 p53和 p185在 52例GCT(GCT按Jaffe分级 :Ⅰ级 15例、Ⅱ级 2 5例、Ⅲ级 12例 )中的表达。结果 11例 p185表达呈阳性 ,阳性率 2 1 2 % ,14例 p53表达阳性 ,阳性率 2 6 9%。其阳性表达与病理分级差异均无显著性 (P >0 0 5)。p53和 p185在复发和无复发的病例中 ,阳性表达率分别为 38 5%、15 4 %和4 6 2 %、2 0 5%。两者同时阳性表达的有 4例 ,p53和p185同时过表达与GCT病理分级无关 (P >0 0 5)而与其复发有显著性差异 (χ2 =6 12 5,P <0 0 5)。结论 p185和p53在GCT中的表达与病理分级无关而与其复发有关。所以 ,我们认为骨巨细胞瘤时 p53和 p185过度表达的临床意义有待进一步研究。

Objective The purpose of this study was to investigate the relationship of p185 and p53 protein overexpression with pathological types and recurrence.Antibodies against p185 and p53 proteins were used to measure expression of these proteins in the nuclei or cell membrane of cells from sections of the giant cell tumor of bone GCT.It showed that 11 out of 52 tumors overexpressed p185 protein and 14 out of 52 tumors had abnormally high levels of p53 protein,4 cases had abnormally high levels of both p185 and p53 proteins,positive expression rates of p185 and p53 in cases with recurrence and cases without reccarrence were 46 2%,20 5% and 38 5%,15 4% respectively.However,there was no association between p185 and p53-positive cases and pathological degree.There was significant correlation between the expression of p185 and p53 protein in the giant cell tumor of bone and recurrence.(χ 2=6 125,P=0 047).However,there was no statitically significance between the expression of p185 and p53 protein in the giant cell tumor of bone and pathological types.So that,we consider that the clinical significance for p185,p53 overexpression in GCT to be researched further.