神经生长因子对部分视神经损伤视网膜神经节细胞继发变性的保护作用

Protection of retinal ganglial cell from secondary degeneration by nerve growth factor in partial optic nerve injury

目的 :在体实验研究神经生长因子 (NGF)对部分视神经损伤的保护效应。方法 :建立视神经不完全损伤模型 ,球后注射神经生长因子 ,记录F VEP ,定量RGCs和髓鞘以评价治疗效果。结果 :与正常组相比 ,NGF组F VEP波形平缓 ,N1和P1潜伏期延长 ,但无显著性差异 ,N1-P1、P1-N1幅值降低、N2 潜伏期延长 ,有显著性差异。NGF对神经节细胞挽救率是19 3%。NGF组未发生溃变的轴突数是损伤未治疗组的 1 83倍。结论 :NGF可挽救视网膜神经节细胞的存活率 ,减少轴突溃变 ,对视网膜神经节细胞继发变性有明显的保护作用。

Objective:To observe in vivo the effects of nerve growth factor(NGF) on the survival of retinal ganglial cell (RGCs) and regeneration of crushed optic nerve axons in adult ratsMethods:After optic nerve crush,NGF was administered by retrobublar injection.The visual evoked potential responses to flash (F-VEP) were recorded,and the numbers of RGCs and axon countedResults:In NGF group,the latent period of F-VEP extended and the swings of F-VEP were depressed,showing no significant difference from normal N 1 and P 1 and distinct difference from normal N 1-P 1、P 1-N 2 and N 2NGF rescue about 193% RGCsNGF prevent axon injury about 183-foldConclusion:The findings of this study demonstrate that treatment with NGF is able to protect neurons and axons from degeneration following optic nerve crush