L-THP对脑缺血大鼠脑组织NO含量变化影响的研究

Experimental Study of the influence of L-THP on NO Content In Focal Ischemic Cerebral Tissue of Rats

目的：通过检测局灶性脑缺血大鼠脑组织中ＮＯ含量的变化及左旋四氢巴马汀（Ｌ－ｔｅｔｒａｂｙｄｒｏｐａｌｍａｔｉｎｅ，Ｌ－ＴＨＰ）的影响．从而探讨Ｌ－ＴＨＰ的脑保护作用机制。方法：采用线栓法制备大鼠右侧大脑中动脉阻断（ＭＣＡＯ）模型。 应用硝酸还原酶法检测缺血３ｈ及４８ｈ脑组织 ＮＯ含量的变化及Ｌ－ＴＨＰ对ＮＯ含量的影响。结果：缺血３ｈ及４８ｈ脑组 织 ＮＯ含量明显升高，且４８ｈ缺血组ＮＯ含量高于３ｈ缺血组。缺血前３０ｍｉｎ应用Ｌ－ＴＨＰ２０ｍｇ／ｋｇ可明显降低３ｈ及 ４８ｈ脑缺血组织中ＮＯ含量。结论：Ｌ－ＴＨＰ可降低局灶性脑缺血脑组织中ＮＯ的含量。其脑保护作用是通过抑制ＮＯ介 导的神经毒性作用实现的。

Objective: To measure the change of nitric oxide (NO) content in focal ischemic cerebral tissue of rats and the influence of L-THP, and to explore the mechanism of protective effect of L-THP. Methods: Cerebral ischemia was achieved by occluding the middle cerebral artery with an intraluminal suture technique in rats. The change of NO content in 3 hours and -48 hours ischemic cerebral tissue and the influence of L-THP was measured by nitrate redutase method. Results: NO content in 3 hours and 48 hours ischemic cerebral tissue was significantly increased, and the group of 48 hours ischemia was higher than 3 hours group. L-THP treated 30 minutes before cerebral ischemia can significantly decrease NO content in ischemic cerebral tissue. Conclusion: L-THP could decrease NO content in focal ischemic cerebral tissue. The mechanism of neuroprotective effect of L-THP was by inhibiting the neurotoxicity of NO-mediated.