IL-1α、IL-1β和TNF-α mRNA在实验性鼠根尖周炎中的表达

IL-1α,IL-1βand TNF-α mRNA Expression in Experimental Periapical Lesions in the Rat

目的 检测IL 1α、IL 1β和TNF αmRNA在鼠根尖周炎组织中的表达 ,了解上述细胞因子与根尖周炎的关系。方法 16只SD大鼠第一、二磨牙开髓 ,分别于术后 3d、7d、14d、2 8d取磨牙根尖周组织 ,提取总RNA ,用RT PCR方法测定IL 1α、IL 1β和TNF αmRNA的表达 ,用看家基因 β Actin作内参照进行半定量分析。 结果 术后 7d鼠根尖周组织中即有IL 1α、TNF α、IL 1βmRNA的表达 ,14d达高峰 ,2 8d有所下降 ,但IL 1α和TNF α的表达量大于IL 1β表达量 ,相差非常显著 (P <0 .0 1)。结论 IL 1α和TNF α可能是导致鼠根尖周炎的主要细胞因子。

Objective IL 1α、IL 1β and TNF α mRNA expression were analyzed in rat experimental periapical lesions,to understand the relationship between the aforementioned cytokines and periapical lesion. Methods The molar tooth pulps of 16 SD rats were surgically exposed and left open to permit infection from the oral environment. Total cell RNA was isolated from periapical tissue obtained on days 3、7、14 and 28 after exposure. mRNA for IL 1α、IL 1β and TNF α was amplified by reverse transcription polymerase chain reaction(RT PCR), and levels were estimated by comparison to the parallel amplification of the housekeeping gene β actin. Results IL 1α、IL 1β and TNF α mRNA were all expressed beginning on day 7, peaked on day 14, and declined somewhat on day 28, but IL 1β mRNA was expressed at much lower levels with the same kinetics( P <0.01). Conclusion IL 1αand TNF α may be primarily cytokines in rat periapical lesion model. [