骨髓基质成骨细胞-松质骨基质复合人工骨肌袋移植的成骨作用

INTRAMUSCULAR BONE FORMATION BY IMPLANTATION OF COMPOSITE MARROW STROMAL OSTEOBLAST-CANCELLOUS BONE MATRIX ARTIFICIAL BONE

成年新西兰兔骨髓细胞体外培养、诱导后 ,经混匀、接种、固化形成骨髓基质成骨细胞 藻酸盐 松质骨基质复合人工骨 ,并植回取材兔肌袋内 ,对照组分别植入藻酸盐 松质骨基质复合物和松质骨基质。植入 4、8周取材 ,行X线摄片、组织学检查 ,观察骨形成情况。结果显示 ,骨髓基质成骨细胞 松质骨基质复合人工骨肌袋内成骨效果明显优于藻酸盐 松质骨基质复合物组和松质骨基质组。复合人工骨标本兼有膜内成骨和软骨成骨 ,以膜内成骨为主 ;对照组仅见少量软骨成骨。提示 ,采用组织工程方法形成的复合人工骨肌袋内成骨作用明显 ,用该方法所形成的组织工程骨展现出良好的应用前景。

The marrow stromal cells of adult New Zealand rabbits cultivated and induced in vitro were used to form MCCAB by mixing,seeding and solidifying methods with the aid of alginate. The MCCABs were auto-transplanted intramuscularly into the rabbits for 4 to 8 weeks. The alginate-cancellous bone matrix composites or the cancellous bone matrix alone were implanted as control. The effectiveness of bone formation was assessed by means of roentgenography and histology.The results showed that the osteogeneses of MCCABs were better than those of the alginate-cancellous bone matrix composites and of the cancellous bone matrix. In the MCCABs, both intramembranous and cartilaginous osteogenesis were seen with the former predominating. In the control, only slight cartilaginous osteogenesis was seen. The results suggested that the osteogenesis of the MCCABs constructed by using tissue engineering method was obvious when transplanted intramuscularly, therefore, this kind of tissue-engineered bone could be an effective way for clinical application.