成骨细胞接种于珊瑚-羟基磷灰石构建骨组织的研究

RESEARCH OF BONE REGENERATION WITH CULTURED OSTEOBLASTS SEEDED IN POROUS CORAL-HYDROXYLAPATITE

体外分离、培养、扩增兔成骨细胞 ,将收集的细胞接种于珊瑚 羟基磷灰石中 ,然后自体移植入兔背部肌肉组织内 ,同时植入未接种细胞的HA一块作为对照。术后 4、8周取材 ,通过组织学、扫描电镜观察新骨的形成情况。结果表明 ,培养的兔成骨细胞在珊瑚 羟基磷灰石表面生长良好。成骨细胞 /珊瑚 羟基磷灰石复合物植入兔背肌肉内 4周 ,局部形成骨样组织 ,8周时形成大量新骨组织。提示珊瑚 羟基磷灰石用于骨组织工程进行骨再造和骨缺损修复有很多优点 ,具有广阔的应用前景。

Rabbit osteoblasts were isolated, expanded in vitro, and then seeded into coral-hydroxylapatite. The osteoblasts/coral-hydroxylapatite composite was were implanted into the muscles of rabbits′ back. Bone regeneration was observed 4, 8 weeks after implantation by histological and scanning electron microscopic examinations.The results showed that osteoblasts could adhere and proliferate well on the surface of coral-hydroxylapatite. 4 weeks after implantation osteoid tissue was observed, 8 weeks after implantation large amount of new bone was found. It suggested that osteoblasts/coral-hydroxylapatite composite may be used for bone tissue engineering.