改善珊瑚表面成骨细胞粘附和增殖的研究

THE STUDY OF IMPROVING THE ADHESION AND PROLIFERATION OF OSTEOBLASTS ON THE SURFACE OF CORAL

新西兰兔骨髓基质成骨细胞作为接种珊瑚的种子细胞。珊瑚块分别用多聚赖氨酸、纤维粘连蛋白(Fn)和单纯培养液处理 ,接种成骨细胞后形成细胞 /珊瑚复合物 ,在体外培养 7、14、2 1天进行观察。电镜显示 ,第7天和 14天 ,Fn处理的珊瑚表面有更多的成骨细胞粘附和骨基质的形成。经多聚赖氨酸处理的珊瑚与培养液处理的珊瑚比较 ,虽然在第 7天通过DNA含量的测定具有较多的细胞数 ,但在第 14、2 1天差异无显著性意义。Fn处理的珊瑚在各时间点细胞数和碱性磷酸酶活性均高于多聚赖氨酸和培养液处理组 ,同时细胞数随培养时间呈增长趋势。

The marrow stromal osteoblasts from New Zealand rabbits were seeded on coral treated with L-polylysine, fibronectin and culture medium only, then the cells/coral composites were cultured in vitro. The cells/coral composites were observed for the process of cells growth and matrix formation at 7, 14 and 21 days after culture. With the aid of electron microscope,it was demonstrated that on the surface of the coral holes,which was treated with fibronectin, there were more adhering osteoblasts and matrix formation than those treated with both L-polylysine and culture medium after 7 and 14 days.Cell count in coral blocks was determined by doing a fluorimetric DNA assay. Although the samples treated with L-polylysine demonstrated higher cell count than the coral treated with culture medium after 7 days, there was no statistically difference between the two after 14, 21 days. At each time point, the samples treated with fibronectin showed higher cell count and alkaline phosphatase activity than the orals otherwise treated, and the cell count also increased with culture time. The study suggests that fibronectin has a significant effect on promoting the adhesion and proliferation of osteoblasts on the surface of coral.