丙型肝炎病毒感染对维持性血液透析患者细胞免疫功能的影响

Effects of hepatitis C virus (HCV) infection on cellular immunity function of patients on maintenance hemodialysis (HD)

目的 探讨丙型肝炎病毒 (HCV )感染对维持性血液透析 (血透 )患者细胞免疫功能的影响。方法 对 6 2例血透者应用酶联免疫吸附法 (ELISA)和逆转录聚合酶链反应 (RT PCR )检测抗 HCV和HCVRNA ,同时应用ELISA法检测血透患者血清sIL 2R ,IL 6和TNF α水平。结果 在 6 2例患者中 ,抗 HCVIgM阳性 2 7例 (43 .6 % ) ,抗 HCVIgG阳性 2 9例 (46 .8% ) ,HCVRNA阳性 34例 (5 4.8% ) ,至少 1项阳性 37例 (5 9.7% )。与对照组相比 ,血透患者血清sIL 2R ,IL 6和TNF α水平均显著增高 (P <0 .0 1) ;与血透患者HCV标志阴性组相比 ,阳性组血清sIL 2R ,IL 6和TNF α水平增高更显著 (P <0 .0 1)。结论 血透患者均存在显著的免疫功能紊乱 ,感染HCV后免疫功能更低下。

Objective To investigate the effect of HCV infection on cellular immunity function of patients on maintenance hemodialysis. Methods ELISA and RT PCR were used to detect serum anti HCV and HCV RNA, ELISA was used to detect serum sIL 2R,IL 6 and TNF α levels in 62 HD patients. Results 27 patients (43.6%) were anti HCV IgM positive, 29 (46.8%) were anti HCV IgG positive, 34 were HCV RNA positive, the total positivity of HCV was 59.7%(37/62), when comparing with the control group, serum sIL 2R, IL 6 and TNF α levels were significantly higher in the HD group ( P <0.01); while comparing with the HCV negative group, the serum sIL 2R, IL 6 and TNF α levels were also significantly higher ( P <0.01).Conclusion The results suggested that cellular immunodeficiency was present in HD patients and was further deteriorated with in positive HCV infection.