肝病患者一氧化氮合酶表达异常及其临床意义

Abnormal expression of nitric oxide synthase and its clinical significance in patients with liver diseases

目的 探讨肝病患者抗氧化能力及一氧化氮合酶 (NOS)表达的临床价值。方法 收集 2 6例急性肝炎(AH )、36例慢性肝炎 (CH)、13例肝炎后肝硬化 (LC)和 2 3例原发性肝癌 (HCC)患者的血清 ,分别检测其总抗氧化能力(TAO)、NOS浓度及一氧化氮 (NO)水平 ,分析它们在肝脏疾病中的改变机制。结果 患者血清TAO在AH、CH中异常率为 80 % ,在LC和HCC为 5 0 % ;血清中NOS活性在肝病患者中的异常率在 70 %左右 ;NO水平在AH、CH和LC中异常率为 70 % ,HCC组为 48%。AH组、CH组TAO平均水平明显高于对照组 ,但LC和HCC组的差异不明显 ;肝病患者NO和NOS平均浓度均显著高于对照组 ,但HCC患者NOS和NO平均浓度均低于AH、CH和LC组患者。结论 肝病患者血清NOS水平与NO浓度呈高度正相关 ,NO增加可能对肝细胞起保护作用。

Objective To explore the clinical significances of total antioxidation (TAO) and nitric oxide aynthase (NOS) expression in patients with liver diseases. Methods The concentrations of TAO, NOS and nitric oxide (NO) were determined and the machanism of their changes were analyzd in sera of patients with acute hepatitis (AH), chronic hepatitis (CH), liver cirrhosis (LC) and hepatocellular carcinoma (HCC).Results The abnormal rate of TAO was 80% in AH or in CH, and 50% in LC or in HCC, respectively. The NOS level over normal reference value was 70% in patients with liver diseases. The abnormal rates of NO level was 70% in AH group, CH group, LC group, and 48% in HCC group, respectively. The average level of serum TAO was significantly higher in AH group and in CH group but not in HCC group and in LC group than that in normal subjects. The average levels of NO and NOS were significantly higher in patients with liver diseases than those in normal subjects, however, they were lower in HCC patients than those in AH group, CH group and LC group, respectively.Conclusions The present data suggest that the level of NOS is positively corrected with NO level in patients with liver diseases, increase of the latter may play a role in protection of hepatocytes.