氧化苦参碱对大鼠肝星状细胞增殖的影响

Effect of oxymatrine on proliferation of hepatic stellate cells of rat liver

目的 探讨氧化苦参碱对大鼠肝星状细胞增殖的影响及其抗肝纤维化的机理。方法 用链酶蛋白酶和胶原酶原位灌流消化正常大鼠肝脏 ,Nycodenz密度梯度离心分离肝星状细胞 ,以MTT比色法观察氧化苦参碱对肝星状细胞毒性作用 ,3H TdR法观察氧化苦参碱对肝星状细胞增殖的效应。结果 氧化苦参碱浓度 >10 -5mol/l时对肝星状细胞增殖有抑制作用 (P <0 .0 5 )。结论 氧化苦参碱可抑制肝星状细胞的增殖 ,有抗肝纤维化的作用。

Objective To investigate the effect of oxymatrine on proliferation of hepatic stellate cells (HSC) of rat liver.Methods HSCs were isolated and cultured from the liver of normal rat by in situ perfusion with pronase and collagenase and density gradient centrifugation with Nycodenz. MTT colorimetry was used for assaying cytotoxicity and incorporation of 3H TdR for assaying proliferation of HSC. Results >10 -5 mol/l oxymatrine can inhibit HSC proliferation ( P <0.05). Conclusion Oxymatrine may prevent hepatic fibrosis by inhibiting HSC proliferation.